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Goat Anti-DYDC1 (aa142-154) Antibody (EB09820)

Related Products: EBP09819 - Immunizing Peptide, EBP09820 - Immunizing Peptide, Goat Anti-DYDC1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB09820 Goat Anti-DYDC1 (aa142-154) Antibody Pep-ELISA, WB-Trf, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09820 1
Lane A - EB09820 (1µg/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10µg protein in RIPA buffer) Lane B - EB09820 (0.1µg/ml) staining of HEK293 mock-transfected lysate (10µg protein in RIPA buffer). Lane C - EB00106 anti-MYC Tag (1/1000) staining HEK293 overexpressing Human DYDC1 lysate (10µg protein in RIPA buffer). Primary incubations were for 1 hour. Detected by chemiluminescence.
EB09820 2
EB09820 (5µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: bA36D19.5, BC019250 protein, DPY30 domain containing 1, DPY30D1, FLJ43920, OTTHUMP00000019963, DYDC1
Official Symbol: DYDC1
Accession Number(s): NP_620167.1
Human GeneID(s): 143241
Non-Human GeneID(s): 69496 (mouse), 688433 (rat)

Immunogen

Peptide with sequence C-TLAEISDRYGAPN, from the internal region of the protein sequence according to NP_620167.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: n transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx. 28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. This 28kDa band is also observed in Human, Mouse and Rat Testis lysates. Recommended concentration: 0.1-1µg/ml.
IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Cow

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