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Goat Anti-E2-230K / UBE2O Antibody (EB05761)

Code Name Applications Availability Size Tested species Price Grade  
EB05761 Goat Anti-E2-230K / UBE2O Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05761 1
EB05761 (4µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2O, ubiquitin-conjugating enzyme E2O, E2-230K, FLJ12878, KIAA1734
Official Symbol: UBE2O
Accession Number(s): NP_071349.3
Human GeneID(s): 63893
Non-Human GeneID(s): 217342 (mouse)

Immunogen

Peptide with sequence ALLEAGMPECTEDK, from the C Terminus of the protein sequence according to NP_071349.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Heart shows textured staining in some myocardial fibres. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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