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Goat Anti-EBF1 / COE1 Antibody (EB07809)

Related Products: EBP07809 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07809 Goat Anti-EBF1 / COE1 Antibody Pep-ELISA, WB, IP Yes 100µg specific antibody in 200µl Human, Mouse $423.00 Elite

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EB07809 1
EB07809 (0.3µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EBF1, early B-cell factor 1, COE1, EBF, O/E-1, OLF1, Collier, Olf and EBF transcription factor 1, early B-cell factor olfactory neuronal transcription factor 1
Official Symbol: EBF1
Accession Number(s): NP_076870.1
Human GeneID(s): 1879
Non-Human GeneID(s): 13591 (mouse), 116543 (rat)
Important Comments: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.

Immunogen

Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1µg/ml.

Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat

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FAQs

In which application has the antibody been tested?


The antibody has been tested in ELISA and WB assays.