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Code Name Applications Availability Size Tested species Price Grade  
EB12263 Goat Anti-EDF1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12263 1
EB12263 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB12263 2
EB12263 (0.3µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: EDF1, endothelial differentiation-related factor 1, EDF-1, MBF1, multiprotein bridging factor 1
Official Symbol: EDF1
Accession Number(s): NP_003783.1; NP_694880.1
Human GeneID(s): 8721
Non-Human GeneID(s): 59022 (mouse), 678956 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003783.1; NP_694880.1).

Immunogen

Peptide with sequence C-NKQHSITKNTAKLDR, from the internal region of the protein sequence according to NP_003783.1; NP_694880.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in lysates of cell line A549 (calculated MW of 16.4kDa according to NP_003783.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Spleen shows nuclear staining of select lymphocytes in the marginal zone. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig

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