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Goat Anti-ELMO3 Antibody (EB05295)

Code Name Applications Availability Size Tested species Price Grade  
EB05295 Goat Anti-ELMO3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05295 1
EB05295 staining (0.1µg/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05295 2
EB05295 (5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ELMO3, engulfment and cell motility 3 (ced-12 homolog, C. elegans), CED12, FLJ13824, ced-12 homolog 3, engulfment and cell motility 3, CED-12, ELMO-3
Official Symbol: ELMO3
Accession Number(s): NP_078988.2
Human GeneID(s): 79767

Immunogen

Peptide with sequence TNFNFCYDCSIAEP, from the C Terminus of the protein sequence according to NP_078988.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 70.0kDa according to NP_ 078988.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: In paraffin embedded Human Prostate shows vesiculate staining in the cytoplasm of secretory cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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