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Goat Anti-EMI2 Antibody (EB06061)

Related Products: EBP06061 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06061 Goat Anti-EMI2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06061 1
EB06061 (0.3µg/ml) staining of Mouse Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FBXO43, EMI2, F-box protein 43, Fbx43, early mitotic inhibitor 2, ERP1
Official Symbol: FBXO43
Accession Number(s): NP_001025031.2
Human GeneID(s): 286151
Non-Human GeneID(s): 78803 (mouse), 315034 (rat)

Immunogen

Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in Mouse Ovary lysates (calculated MW of 78.4kDa according to NP_001025031.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human, Rat and Pig Testis, and Human Ovary for this particular batch.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Specific References

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Pasternak M, Pfender S, Santhanam B, Schuh M
The BTG4 and CAF1 complex prevents the spontaneous activation of eggs by deadenylating maternal mRNAs.
Open Biol. 2016 Sep;6(9)
PMID: 27605379

This antibody (previous batch) has been successfully used in Western blot on Mouse:
Bernhardt ML, Kong BY, Kim AM, O'Halloran TV, Woodruff TK.
A zinc-dependent mechanism regulates meiotic progression in mammalian oocytes.
Biol Reprod. 2012 Apr 19;86(4):114.
PMID: 22302686

This antibody (previous batch) has been successfully used in Western blot, Immunocytochemistry and Flow Cytometry on Rat:
Perrard MH, Chassaing E, Montillet G, Sabido O, Durand P.
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and β-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes.
PLoS One. 2009 Oct 5;4(10):e7237.
PMID: 19802389

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