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Code Name Applications Availability Size Tested species Price Grade  
EB11694 Goat Anti-Endophilin-A1 / SH3GL2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11694 1
EB11694 (0.3µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11694 2
EB11694 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: bA335L15.1 (SH3-domain GRB2-like 2), CNSA2, EEN-B1, endophilin, Endophilin A1 BAR domain, endophilin-1, FLJ20276, FLJ25015, OTTHUMP00000021084, SH3 domain protein 2A, SH3 domain-containing GRB2-like protein 2, SH3D2A, SH3-domain GRB2-like 2, SH3GL2, SH3P4
Official Symbol: SH3GL2
Accession Number(s): NP_003017.1
Human GeneID(s): 6456

Immunogen

Peptide with sequence C-SLEFPTGDSTQPN, from the internal region of the protein sequence according to NP_003017.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.0kDa according to NP_003017.1). Recommended concentration: 0.3-1µg/ml.
IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in some but not all neuronal cells. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig

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