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Goat Anti-Endophilin-A2 Antibody (EB10691)

Related Products: EBP10691 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10691 Goat Anti-Endophilin-A2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10691 1
EB10691 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB10691 2
EB10691 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: SH3GL1, SH3-domain GRB2-like 1, CNSA1, EEN, MGC111371, SH3D2B, SH3P8, EEN fusion partner of MLL, SH3 domain GRB2-like 1, SH3 domain-containing GRB2-like protein 1, SH3-containing Grb-2-like 1 protein, SH3-containing protein EEN, endophilin-A2, endophilin-2, SH3 domain protein 2B, extra 11-19 leukemia fusion, extra eleven-nineteen leukemia fusion gene protein
Official Symbol: SH3GL1
Accession Number(s): NP_003016.1
Human GeneID(s): 6455

Immunogen

Peptide with sequence C-EPFDLGEPEQSN, from the internal region of the protein sequence according to NP_003016.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in lysates of cell line K562 (calculated MW of 41.5kDa according to NP_003016.1. The observed molecular weight corresponds to earlier findings in literature with different antibodies (So et al, Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2563-8.; PMID: 9122235). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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