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Goat Anti-ENPP1 / PC1 Antibody (EB06948)

Code Name Applications Availability Size Tested species Price Grade  
EB06948 Goat Anti-ENPP1 / PC1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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EB06948 1
EB06948 (8µg/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06948 2
EB06948 Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1
Official Symbol: ENPP1
Accession Number(s): NP_006199.2
Human GeneID(s): 5167

Immunogen

Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Thyroid Gland. Recommended concentration: 8µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

Specific References

This antibody (previous batch) has been successfully used in ICC on Mouse:
Tomomaya Yamamoto et al.
Comparative immunolocalization of tissue nonspecific alkaline phosphatase and ectonucleotide pyrophosphatase/phosphodiesterase 1 in murine bone
Journal of Oral Biosciences (2021), https://doi.org/10.1016/j.job.2021.08.001
PMID: 0

This antibody (previous batch) has been successfully used in Western Blot and IHC:
Lau WM, Doucet M, Stadel R, Huang D, Weber KL, Kominsky SL
Enpp1: a potential facilitator of breast cancer bone metastasis.
PLoS One. 2013 Jul 5;8(7):e66752.
PMID: 23861746

This antibody (previous batch) has been successfully used in WB:
Simão AM, Yadav MC, Narisawa S, Bolean M, Pizauro JM, Hoylaerts MF, Ciancaglini P, Millán JL.
Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics.
J Biol Chem. 2010 Mar 5;285(10):7598-609.
PMID: 20048161

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