Change country
Home

Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody (EB08135)

Related Products: EBP08135 - Immunizing Peptide, EBP08171 - Immunizing Peptide, Goat Anti-EPB41L2 / 4.1G (aa 347 to 357) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08135 Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody Pep-ELISA, IF, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08135 1
EB08135 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08135 2
EB08135 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cell junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08135 3
EB08135 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB08135 4
EB08135 (2.5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08135 5
EB08135 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2
Official Symbol: EPB41L2
Accession Number(s): NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1
Human GeneID(s): 2037
Important Comments: This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1 (iso i), NP_001337243.1 (iso j), NP_001337249.1 (iso k).

Immunogen

Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary testing showed bands at approx 110+40kDa in Human Cerebellum lysatesafter 0.1µg/ml antibody staining (calculated MW of 113kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Product Reviews

Please login to review this product.