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Goat Anti-EPI64/TBC1D10A Antibody (EB06192)

Related Products: EBP06192 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06192 Goat Anti-EPI64/TBC1D10A Antibody Pep-ELISA, IF, FC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06192 1
EB06192 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB06192 2
EB06192 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06192 3
EB06192 (4.75µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06192 4
EB06192 (4.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: EPI64, EBP50-PDZ interactor of 64 kD, Rab27A-GAP-alpha, EPI 64, Dj130h16, TBC1 domain family member 10A, TBC1D10, TBC1 domain family member 10A
Official Symbol: TBC1D10A
Accession Number(s): NP_114143; NP_001191169.1
Human GeneID(s): 83874

Immunogen

Peptide with sequence AKSNGENGPRAPA-C, from the N Terminus of the protein sequence according to NP_114143; NP_001191169.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ plasma membranes and nuclei of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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