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Goat Anti-ERBB3 / HER3 Antibody - Trial (EB06903-T)

Related Products: EBP06903 - Immunizing Peptide, Goat Anti-ERBB3 / HER3 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06903-T Goat Anti-ERBB3 / HER3 Antibody - Trial Pep-ELISA, FC, IF, IHC Yes 20µg specific antibody in 40µl Human $105.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB06903-T 1
EB06903 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06903-T 2
EB06903 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB06903-T 3
EB06903 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ERBB3, HER3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), HGNC:3431, ErbB-3, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, erbB-3, LCCS2, MGC88033, receptor tyrosine-protein kinase erbB-3, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Official Symbol: ERBB3
Accession Number(s): NP_001973.2
Human GeneID(s): 2065
Non-Human GeneID(s): 13867 (mouse), 29496 (rat)
Important Comments: This antibody is expected to recognise isoform 1 (NP_001973.2) only.

Immunogen

Peptide with sequence C-HSRLFPKANAQRT, from the C Terminus of the protein sequence according to NP_001973.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
IHC: Paraffin embedded Human Lung. Recommend concentration: 5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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