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Goat Anti-ERP29 Antibody (EB08977)

Code Name Applications Availability Size Tested species Price Grade  
EB08977 Goat Anti-ERP29 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Rat $341.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB08977 1
EB08977 (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ERP29, endoplasmic reticulum protein 29, C12orf8, ERp28, ERp31, PDI-DB, endoplasmic reticulum lumenal protein ERp28
Official Symbol: ERP29
Accession Number(s): NP_006808.1
Human GeneID(s): 10961
Non-Human GeneID(s): 67397 (mouse), 117030 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_006808.1) only.

Immunogen

Peptide with sequence C-EKNKMSDGKKEELQ, from the C Terminus of the protein sequence according to NP_006808.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx. 28kDa band observed in Human Liver lysates (calculated MW of 29.0kDa according to NP_006808.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. This product has been successfully used in WB on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.

Immunofluorescence:This product has been successfully used in IF on Rat: Gao J et al, Mol Med Rep. 2016 Jan;13(1):398-402. PMID: 26548430.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow

Specific Reference

This antibody has been successfully used in WB and IF on Rat:
Gao J, Zhang Y, Wang L, Xia L, Lu M, Zhang B, Chen Y, He L.
Endoplasmic reticulum protein 29 is involved in endoplasmic reticulum stress in islet beta cells.
Mol Med Rep. 2016 Jan;13(1):398-402.
PMID: 26548430

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