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|EB08050||Goat Anti-Factor XIIIa (49-62) Antibody||Pep-ELISA, IHC||100µg specific antibody in 200µl||Human||$333.00|
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Peptide with sequence C-TSVHLFKERWDTNK, from the internal region of the protein sequence according to NP_000120.2.
Please note the peptide is available for sale.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Q: the epitope of your antibody is aa49-62 of the FXIII A-subunit. Is this a neoepitope which is only exposed upon thrombin cleavage of FXIII? If that is the case, does your antibody specifically recognise ONLY activated FXIIIa and does not cross-react with the non-activated FXIII zymogen?
A: According to Uniprot is the propeptide to residue 38. Our antibody therefore will react to the N-terminal part of the activated protein, but it will obviously also recognize the precursor in denatured conditions.