Change country
Home

Goat Anti-FANCF (aa217-228) Antibody (EB10140)

Related Products: EBP06112 - Immunizing Peptide, EBP10140 - Immunizing Peptide, Goat Anti-FANCF Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10140 Goat Anti-FANCF (aa217-228) Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10140 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with EB10140 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10140 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FAF, Fanconi anemia, complementation group F, MGC126856, FANCF
Official Symbol: FANCF
Accession Number(s): NP_073562.1
Human GeneID(s): 2188

Immunogen

Peptide with sequence C-RPQEELEPGIHK, from the internal region of the protein sequence according to NP_073562.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: In transfected HEK293 transiently expressing full-length Human FANCF (myc and DYKDDDDK tagged), a band of approx. 40kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Mu-Yan Cai, Connor E. Dunn, Wenxu Chen, Bose S. Kochupurakkal, Huy Nguyen, Lisa A. Moreau, Geoffrey I. Shapiro, Kalindi Parmar, David Kozono, and Alan D. D’Andrea
Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection.
Cell Rep. 2020 Feb 18; 30(7): 2402-2415.e5.
PMID: 32075772

Product Reviews

Please login to review this product.