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Goat Anti-FANCF (aa217-228) Antibody (EB10140)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB10140 | Goat Anti-FANCF (aa217-228) Antibody | Pep-ELISA, WB-Trf |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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Target Protein
Principal Names: FAF, Fanconi anemia, complementation group F, MGC126856, FANCFOfficial Symbol: FANCF
Accession Number(s): NP_073562.1
Human GeneID(s): 2188
Immunogen
Peptide with sequence C-RPQEELEPGIHK, from the internal region of the protein sequence according to NP_073562.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:16000.Western blot: In transfected HEK293 transiently expressing full-length Human FANCF (myc and DYKDDDDK tagged), a band of approx. 40kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human
Specific Reference
This antibody has been successfully used in the following paper:
Mu-Yan Cai, Connor E. Dunn, Wenxu Chen, Bose S. Kochupurakkal, Huy Nguyen, Lisa A. Moreau, Geoffrey I. Shapiro, Kalindi Parmar, David Kozono, and Alan D. D’Andrea
Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection.
Cell Rep. 2020 Feb 18; 30(7): 2402-2415.e5.
PMID: 32075772