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Goat Anti-Fascin 2 Antibody (EB08002)

Related Products: EBP08002 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08002 Goat Anti-Fascin 2 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

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ARP American Research Products, Inc
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EB08002 1
EB08002 (4µg/ml) staining of paraffin embedded Mouse Brain. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), RFSN, RP30, fascin 2, retinal fascin
Official Symbol: FSCN2
Accession Number(s): NP_001070650.1; NP_036550.1
Human GeneID(s): 25794
Non-Human GeneID(s): 238021 (mouse), 303741 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).

Immunogen

Peptide with sequence CHHRGSNQLDTNR, from the internal region of the protein sequence according to NP_001070650.1; NP_036550.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
IHC: Paraffin embedded Mouse Brain. Recommended concentration: 4µg/ml. This product was successfully used on sections of mouse cochlea as descibed in Shin et al, J Neurosci. 2010 Jul 21;30(29):9683-94; PMID: 20660251.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific References

This antibody has been successfully used in the following papers:
Shin JB, Longo-Guess CM, Gagnon LH, Saylor KW, Dumont RA, Spinelli KJ, Pagana JM, Wilmarth PA, David LL, Gillespie PG, Johnson KR.
The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice.
J Neurosci. 2010 Jul 21;30(29):9683-94.
PMID: 20660251

Ramakrishnan NA, Drescher MJ, Khan KM, Hatfield JS, Drescher DG.
HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2.
J Biol Chem. 2012 Nov 2;287(45):37628-46.
PMID: 22948144

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