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Goat Anti-FBXL3 Antibody (EB07392)

Code Name Applications Availability Size Tested species Price Grade  
EB07392 Goat Anti-FBXL3 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07392 1
EB07392 (1µg/ml) staining of Human Adipose lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07392 2
EB07392 (5µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Target Protein

Principal Names: FBXL3, F-box and leucine-rich repeat protein 3, FBL3, FBL3A, FBXL3A, F-box and leucine-rich repeat protein 3A, F-box protein Fbl3a
Official Symbol: FBXL3
Accession Number(s): NP_036290.1
Human GeneID(s): 26224

Immunogen

Peptide with sequence KRGGRDSDRNSSEE-C, from the N Terminus of the protein sequence according to NP_036290.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50-55kDa band observed in Human Adipose lysates (calculated MW of 48.7kDa according to NP_036290.1). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Thyroid. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig