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Goat Anti-FCGR1A/CD64 Antibody (EB12555)

Related Products: EBP12555 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB12555 Goat Anti-FCGR1A/CD64 Antibody Pep-ELISA, WB, FC Allow up to 10 days for delivery 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12555 1

EB12555 optimised QC. Primary incubation 1 hour at room temperature.

Image A: U937 cell lysate at primary Ab concentration 1ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB12555 2

EB12555 optimised QC. Primary incubation 1 hour at room temperature.

Images A+B: Human Liver lysate + peptide at primary Ab concentration 1.5ug/ml, Images C+D: Human Tonsil lysate 1 + peptide at primary Ab concentration 1ug/ml, Image E: Human Tonsil lysate 2 at primary Ab concentration 1ug/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB12555 3
EB12555 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FCER1A, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, FCE1A, FcERI, Fc IgE receptor, alpha polypeptide, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, igE Fc receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
Official Symbol: FCGR1A
Accession Number(s): NP_000557.1; NP_001365740.1; NP_001365737.1
Human GeneID(s): 2209

Immunogen

Peptide with sequence C-SKTLRGRNTSSEYQ, from the internal region of the protein sequence according to NP_000557.1; NP_001365740.1; NP_001365737.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 45kDa band observed in lysates of cell line U937, approx. 48+55kDa in Human Liver lysates, and approx. 28+48kDa in Human Tonsil lysates (calculated MW of 42.6kDa according to NP_000557.1 and 31.0kDa according to NP_001365740.1; NP_001365737.1). All bands were successfully blocked by incubation with the immunizing peptide Recommended concentration: 0.01-0.5µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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