Change country
Home

Goat Anti-Fgf14 (mouse N terminus) Antibody (EB11834)

Related Products: EBP11834 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11834 Goat Anti-Fgf14 (mouse N terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11834 1
EB11834 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11834 2
EB11834 (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0.5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
Click here to download PDF datasheet.

Target Protein

Principal Names: Fgf14, FGF-14, Fhf4, FHF-4, fibroblast growth factor 14, fibroblast growth factor homologous factor 4, mFHF-4(1B)
Official Symbol: Fgf14
Accession Number(s): NP_034331.2; NP_997550.1
Human GeneID(s): 2259
Non-Human GeneID(s): 14169 (mouse), 63851 (rat)
Important Comments: This antibody is expected to recognize mouse isoform a (NP_034331.2) and human isoform 1A (NP_004106.1).

Immunogen

Peptide with sequence REQHWDRPSASR-C, from the N Terminus of the protein sequence according to NP_034331.2; NP_997550.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Product Reviews

Please login to review this product.