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Goat Anti-Fgf14 (mouse N terminus) Antibody (EB11834)

Related Products: EBP11834 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11834 Goat Anti-Fgf14 (mouse N terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11834 1
EB11834 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11834 2
EB11834 (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0.5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Éva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.
Click here to download PDF datasheet.

Target Protein

Principal Names: Fgf14, FGF-14, Fhf4, FHF-4, fibroblast growth factor 14, fibroblast growth factor homologous factor 4, mFHF-4(1B)
Official Symbol: Fgf14
Accession Number(s): NP_034331.2; NP_997550.1
Human GeneID(s): 2259
Non-Human GeneID(s): 14169 (mouse), 63851 (rat)
Important Comments: This antibody is expected to recognize mouse isoform a (NP_034331.2) and human isoform 1A (NP_004106.1).

Immunogen

Peptide with sequence REQHWDRPSASR-C, from the N Terminus of the protein sequence according to NP_034331.2; NP_997550.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
IHC: Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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