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Goat Anti-fibrinogen beta chain Antibody (EB12379)

Code Name Applications Availability Size Tested species Price Grade  
EB12379 Goat Anti-fibrinogen beta chain Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB12379 1
EB12379 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FGB, fibrinogen beta chain, fibrinogen, B beta polypeptide
Official Symbol: FGB
Accession Number(s): NP_005132.2; NP_001171670.1
Human GeneID(s): 2244
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005132.2; NP_001171670.1).

Immunogen

Peptide with sequence C-RSKIQKLESDVSAQ, from the internal region of the protein sequence according to NP_005132.2; NP_001171670.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 55.9kDa according to NP_005132.2). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog