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Goat Anti-Fibroblast growth factor 21 Antibody (EB08067)

Related Products: EBP08067 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08067 Goat Anti-Fibroblast growth factor 21 Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc
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EB08067 1
EB08067 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08067 2
EB08067 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing ER and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: fibroblast growth factor 21, FGF21
Official Symbol: FGF21
Accession Number(s): NP_061986.1
Human GeneID(s): 26291

Immunogen

Peptide with sequence CQRPDGALYGSLH, from the internal region of the protein sequence according to NP_061986.1 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.

Immunofluorescence: Expression of the protein seen in the Endoplasmic reticulum and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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