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Code Name Applications Availability Size Tested species Price Grade  
EB05449 Goat Anti-Flotillin 1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05449 1
EB05449 (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05449 2
EB05449 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05449 3
EB05449 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2
Official Symbol: FLOT1
Accession Number(s): NP_005794.1; NP_001305804.1
Human GeneID(s): 10211
Non-Human GeneID(s): 14251 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).

Immunogen

Peptide with sequence C-SISQVNHKPLRTA, from the C Terminus of the protein sequence according to NP_005794.1; NP_001305804.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in lysates of cell line K562 and in preliminary testing of cell lysate MCF7 (calculated MW of 47.4kDa according to NP_005794.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig, Cow

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