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Goat Anti-Flotillin 2 / FLOT2 (C Term) Antibody (EB05448)

Related Products: EBP05448 - Immunizing Peptide, EBP07743 - Immunizing Peptide, Goat Anti-Flotillin 2 / FLOT2 (internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05448 Goat Anti-Flotillin 2 / FLOT2 (C Term) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05448 1
EB05448 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05448 2
EB05448 (3.75µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05448 3
EB05448 (3.75µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FLOT2, flotillin 2, reggie-1, ESA, ESA1, M17S1, Flotillin 2 (epidermal surface antigen 1), ECS-1, ECS1, membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1)
Official Symbol: FLOT2
Accession Number(s): NP_004466.2
Human GeneID(s): 2319

Immunogen

Peptide with sequence C-SKIPLIKKATGVQV, from the C Terminus of the protein sequence according to NP_004466.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in Human Brain (Cerebellum), Lung and Testis lysates (calculated MW of 47.1kDa according to NP_004466.2). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Tonsil and Kidney. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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