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Code Name Applications Availability Size Tested species Price Grade  
EB07868 Goat Anti-FMR1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07868 1
EB07868 (0.1µg/ml) staining of KELLY cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB07868 2
EB07868 (2.5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: FMR1, fragile X mental retardation 1, FMRP, FRAXA, MGC87458
Official Symbol: FMR1
Accession Number(s): NP_002015.1
Human GeneID(s): 2332

Immunogen

Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C Terminus) of the protein sequence according to NP_002015.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in lysates of cell line KELLY (calculated MW of 71.2kDa according to NP_002015.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sittler et al, Hum Mol Genet. 1996 Jan;5(1):95-102; PMID: 8789445). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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