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Code Name Applications Availability Size Tested species Price Grade  
EB06006-T Goat Anti-FOXC1 (C Term) Antibody - Trial Pep-ELISA, IF, FC Yes 20µg specific antibody in 40µl Human $105.00 Elite

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Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
TestShot
EB06006-T 1
EB06006 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06006-T 2
EB06006 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IRID1, FREAC3, iridogoniodysgenesis type 1, forkhead (Drosophila)-like 7, forkhead-related activator 3, Forkhead, drosophila, homolog-like 7, FREAC-3, RIEG3, forkhead/winged helix-like transcription factor 7, myeloid factor-delta
Official Symbol: FOXC1
Accession Number(s): NP_001444.2
Human GeneID(s): 2296
Non-Human GeneID(s): 17300 (mouse)

Immunogen

Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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