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Code Name Applications Availability Size Tested species Price Grade  
EB11526 Goat Anti-Furin (C terminus) Antibody Pep-ELISA, WB-Trf Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB11526 1
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with EB11526 (0.01ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB11526 (0.01mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: dibasic processing enzyme, dibasic-processing enzyme, FES upstream region, FUR, FURIN, furin (paired basic amino acid cleaving enzyme), furin, membrane associated receptor protein, OTTHUMP00000194747, PACE, paired basic amino acid residue-cleaving enzyme, PCSK3, proprotein convertase subtilisin/kexin type 3, SPC1
Official Symbol: FURIN
Accession Number(s): NP_002560.1
Human GeneID(s): 5045
Non-Human GeneID(s): 18550 (mouse), 54281 (rat)

Immunogen

Peptide with sequence C-EDEGRGERTAFIKDQ, from the C Terminus of the protein sequence according to NP_002560.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx. 110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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