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Goat Anti-G6PD (aa 308 - 320) Antibody (EB07841)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB07841 | Goat Anti-G6PD (aa 308 - 320) Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $411.00 |  |
|
Ordering Information forOrder direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. Order offline99% of products are held in stock for immediate shipment, see individual product pages for availability. Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:
In stock orders received by 11 am PST on Monday through Friday are shipped the same day. Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. |
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Target Protein
Principal Names: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PDOfficial Symbol: G6PD
Accession Number(s): NP_000393.4 ; NP_001035810.1
Human GeneID(s): 2539
Non-Human GeneID(s): 14381 (mouse), 24377 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
Immunogen
Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 ; NP_001035810.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 55-60kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5kDa according to NP_000393.4 and 59.3kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5µg/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog
Specific References
This antibody has been successfully used in the following paper:
Adriana Ann Garcia, Irimpan I. Mathews, Naoki Horikoshi, Tsutomu Matsui, Manat Kaur, Soichi Wakatsuki, and Daria Mochly-Rosen
Stabilization of glucose-6-phosphate dehydrogenase oligomers enhances catalytic activity and stability of clinical variants.
J Biol Chem. 2022 Mar; 298(3): 101610.
PMID: 35065072
This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371
Product Reviews
Please login to review this product.- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol