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Goat Anti-GAD2 / GAD65 Antibody (EB06730)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB06730 | Goat Anti-GAD2 / GAD65 Antibody | Pep-ELISA, WB, IHC |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest |
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EB06730 (0.5µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06730 (6µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06730 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Target Protein
Principal Names: GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas)Official Symbol: GAD2
Accession Number(s): NP_000809.1; NP_001127838.1
Human GeneID(s): 2572
Important Comments: This antibody is expected to recognise both reported variants.
Immunogen
Peptide with sequence C-TLEDNEERMSRLSK, from the internal region of the protein sequence according to NP_000809.1; NP_001127838.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.Western blot: Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 6-8µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog