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Goat Anti-GAD2 / GAD65 Antibody (EB06730)

Code Name Applications Availability Size Tested species Price Grade  
EB06730 Goat Anti-GAD2 / GAD65 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $315.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Now shipping direct to US customers! 99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email (usasales@everestbiotech.com), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB06730 1
EB06730 (0.5µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06730 2
EB06730 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB06730 3
EB06730 (2.5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: GAD2, GAD65, glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2 (pancreatic islets and brain, 65kD), RP11-420F12.2, MGC161605, MGC161607, Glutamate decarboxylase-2 (pancreas)
Official Symbol: GAD2
Accession Number(s): NP_000809.1; NP_001127838.1
Human GeneID(s): 2572
Important Comments: This antibody is expected to recognise both reported variants.

Immunogen

Peptide with sequence C-TLEDNEERMSRLSK, from the internal region of the protein sequence according to NP_000809.1; NP_001127838.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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