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Goat Anti-C2GnT-M (aa 410 to 422) Antibody (EB08256)

Related Products: EBP08256 - Immunizing Peptide, EBP08257 - Immunizing Peptide, Goat Anti-C2GnT-M (aa 273 to 284) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB08256 Goat Anti-C2GnT-M (aa 410 to 422) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08256 1
EB08256 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB08256 2
HEK293 overexpressing GCNT3 (RC202007) and probed with EB08256 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: GCNT3, glucosaminyl (N-acetyl) transferase 3, mucin type, C2/4GnT, C2GnT-M, C2GnT2, GnT-M, beta1,6-N-acetylglucosaminyltransferase
Official Symbol: GCNT3
Accession Number(s): NP_004742.1
Human GeneID(s): 9245

Immunogen

Peptide with sequence C-NKFDPKVDDNALQ, from the internal region (near C terminus) of the protein sequence according to NP_004742.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55-60kDa band observed in Human Duodenum lysates (calculated MW of 50.9kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

Specific Reference

Petrosyan A, Ali MF, Verma SK, Cheng H, Cheng PW.
Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail.
Int J Biochem Cell Biol. 2012 Jul;44(7):1153-65.
PMID: 22525330

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