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Goat Anti-GFAP Antibody - Trial (EB07478-T)

Related Products: EBP07478 - Immunizing Peptide, Goat Anti-GFAP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07478-T Goat Anti-GFAP Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse, Rat $105.00 Elite_Plus

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB07478-T 1

EB07478 optimised QC. Primary incubation 1 hour at room temperature.

Image A: Human Cerebral Cortex lysate at primary Ab concentration 0.001µg/ml. Image B: Mouse Brain lysate at primary Ab concentration 0.03ug/ml. Image C: Rat Brain lysate at primary Ab concentration 0.005µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GFAP, glial fibrillary acidic protein, FLJ45472, intermediate filament protein
Official Symbol: GFAP
Accession Number(s): NP_002046.1
Human GeneID(s): 2670
Non-Human GeneID(s): 14580 (mouse), 24387 (rat)
Important Comments: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Immunogen

Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 48kDa band observed in Human Cerebral Cortex lysates, and approx. 50kDa in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration: 0.001-0.03µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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