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Goat Anti-GFAP Antibody - Trial (EB07478-T)

Related Products: EBP07478 - Immunizing Peptide, Goat Anti-GFAP Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07478-T Goat Anti-GFAP Antibody - Trial Pep-ELISA, WB Yes 20µg specific antibody in 40µl Human, Mouse, Rat $105.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
TestShot
EB07478-T 1
EB07478 (0.001µg/ml) staining of Human Cerebral Cortex (A), (0.03ug/ml) Mouse Brain (B) and (0.005ug/ml) Rat Brain (C) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GFAP, glial fibrillary acidic protein, FLJ45472, intermediate filament protein
Official Symbol: GFAP
Accession Number(s): NP_002046.1
Human GeneID(s): 2670
Non-Human GeneID(s): 14580 (mouse), 24387 (rat)
Important Comments: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

Immunogen

Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 48kDa band observed in Human Cerebral Cortex lysates, and approx. 50kDa in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration: 0.001-0.03µg/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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