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Goat Anti-GLI1 Antibody (EB07027)

Related Products: EBP07027 - Immunizing Peptide, EBP10594 - Immunizing Peptide, Goat Anti-GLI1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB07027 Goat Anti-GLI1 Antibody Pep-ELISA, IF Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07027 1
EB07027 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Target Protein

Principal Names: GLI1, glioma-associated oncogene homolog 1 (zinc finger protein), HGNC:4317, GLI, glioma-associated oncogene homolog (zinc finger protein), glioma-associated oncogene homolog 1, Tax Helper Protein, GLI family zinc finger 1, glioma-associated oncogene family zinc finger 1, glioma-associated oncogene homolog 1 (zinc finger protein)
Official Symbol: GLI1
Accession Number(s): NP_005260.1; NP_001153517.1; NP_001161081.1
Human GeneID(s): 2735

Immunogen

Peptide with sequence C-EDAPAQEPSYQSPK, from the internal region of the protein sequence according to NP_005260.1; NP_001153517.1; NP_001161081.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human