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Goat Anti-GLP1R Antibody (EB11676)

Related Products: EBP11676 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11676 Goat Anti-GLP1R Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human, Rat, Pig $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11676 1
EB11676 (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11676 2
EB11676 (1µg/ml) staining of Rat (A) and (0.1ug/ml) Pig (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11676 3
EB11676 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB11676 4
EB11676 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GLP-1 receptor, Glp1r, GLP-1R, GLP-1-R, GLP1Rc, glucagon-like peptide 1 receptor
Official Symbol: GLP1R
Accession Number(s): NP_002053.3
Human GeneID(s): 2740
Non-Human GeneID(s): 14652 (mouse), 25051 (rat)
Important Comments: The immunizing peptide represents part of an extracellular region.

Immunogen

Peptide with sequence C-QHQWDGLLSYQD, from the internal region of the protein sequence according to NP_002053.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 55kDa band observed in Human Hippocampus and in Rat and Pig Brain lysates (calculated MW of 53.0kDa according to Human NP_002053.3, 52.9kDa according to Rat NP_036860.1 and 52.7kDa according to Pig NP_001243523.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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