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Goat Anti-GLuR5 / GRIK1 Antibody (EB08000)

Related Products: EBP08000 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08000 Goat Anti-GLuR5 / GRIK1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08000 1
EB08000 (1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08000 2
EB08000 (3.75µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GLUR5, GRIK1, glutamate receptor, ionotropic, kainate 1, EAA3, EEA3, GLR5, excitatory amino acid receptor 3
Official Symbol: GRIK1
Accession Number(s): NP_000821.1; NP_783300.1
Human GeneID(s): 2897
Non-Human GeneID(s): 14805 (mouse), 29559 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.

Immunogen

Peptide with sequence QCKQTHPTNSTS, from the internal region (near the C Terminus) of the protein sequence according to NP_000821.1; NP_783300.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Christensen et al , J Neurosci. 2004 Oct 13;24(41):8986-93.; PMID: 15483117). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. An additional very strong band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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