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Goat Anti-Glutamate Dehydrogenase Antibody (EB09856)

Related Products: EBP09856 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09856 Goat Anti-Glutamate Dehydrogenase Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $450.00 Elite_Plus

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EB09856 1
EB09856 (0.03µg/ml) staining of Human (A) and Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09856 2
EB09856 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB09856 3
EB09856 (3.75µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GDH, GDH1, GLUD, glutamate dehydrogenase 1, MGC132003, GLUD1
Official Symbol: GLUD1 / GLUD2
Accession Number(s): NP_005262.1; NP_036216.2
Human GeneID(s): 2746 , 2747
Non-Human GeneID(s): 14661 (mouse), 24399 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

Immunogen

Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5µg/ml.

Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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