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Goat Anti-GNAQ / ALPHA-q (aa162-175) Antibody - Trial (EB10742-T)

Related Products: EBP10742 - Immunizing Peptide, Goat Anti-GNAQ / ALPHA-q (aa162-175) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB10742-T Goat Anti-GNAQ / ALPHA-q (aa162-175) Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Pig $105.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB10742-T 1
EB10742 (0.1µg/ml) staining of Mouse (A) and Rat (B) Testis lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10742-T 2
EB10742 (0.1µg/ml) staining of Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10742-T 3
EB10742 (0.1µg/ml) staining of Pig Colon (A) and Pig Spleen (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10742-T 4
EB10742 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: GNAQ, guanine nucleotide binding protein (G protein), q polypeptide, G-ALPHA-q, GAQ
Official Symbol: GNAQ
Accession Number(s): NP_002063.2
Human GeneID(s): 2776
Non-Human GeneID(s): 14682 (mouse), 81666 (rat)

Immunogen

Peptide with sequence C-NDLDRVADPAYLPT, from the internal region of the protein sequence according to NP_002063.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40kDa band observed in Human, Mouse and Rat Testis lysates, in Pig Colon and Spleen lysates and in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 42.1kDa according to Human NP_002063.2 and to Rat NP_112298.1 ). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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