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Goat Anti-GPR94 / TRA1 Antibody (EB07290)

Related Products: EBP07290 - Immunizing Peptide, Goat Anti-GPR94 / TRA1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB07290 Goat Anti-GPR94 / TRA1 Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07290 1
EB07290 (0.5µg/ml) staining of HeLa (A) and U2OS (B), and (0.3ug/ml) on HepG2 (C), cell lysate (35µg protein in RIPA buffer). Detected by chemiluminecence.
EB07290 2
EB07290 (6µg/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB07290 3
EB07290 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
EB07290 4
EB07290 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRA1, GRP94, tumor rejection antigen (gp96) 1, HGNC:12028, ECGP, GP96, Tumor rejection antigen-1 (gp96), endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, tumor rejection antigen (gp96) 1
Official Symbol: HSP90B1
Accession Number(s): NP_003290.1
Human GeneID(s): 7184
Non-Human GeneID(s): 22027 (mouse), 362862 (rat)

Immunogen

Peptide with sequence C-KEGVKFDESEKTKE, from the internal region of the protein sequence according to NP_003290.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 100kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS and in preliminary testing of NIH3T3 cell lysate and Mouse Liver lysate (calculated MW of 92.5kDa according to Human NP_003290.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-0.5µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.
IHC: Paraffin embedded Human Liver. Recommended concentration: 5-7µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

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