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Goat Anti-GRB2, biotinylated Antibody (EB05284-B)

Code Name Applications Availability Size Tested species Price Grade  
EB05284-B Goat Anti-GRB2, biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05284-B 1
Biotinylated EB05284 (0.3µg/ml) staining of MOLT4 (A) and Human Thymus (B) lysates (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRB2, growth factor receptor-bound protein 2, ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, HT027, SH2/SH3 adapter GRB2, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, protein Ash
Official Symbol: GRB2
Accession Number(s): NP_002077.1; NP_987102.1
Human GeneID(s): 2885
Non-Human GeneID(s): 14784 (mouse), 81504 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Immunogen

Peptide with sequence C-PRNYVTPVNRNV., from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in lysates of Human Thymus and of cell line MOLT4 (calculated MW of 25.2kDa according to NP_002077.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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