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Code Name Applications Availability Size Tested species Price Grade  
EB05284-T Goat Anti-GRB2 Antibody - Trial Pep-ELISA, WB, IHC Yes 20µg specific antibody in 40µl Human, Mouse, Rat, Pig $105.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
TestShot
EB05284-T 1
EB05284 (0.05µg/ml) staining of Human Thymus (A) and MOLT4 (B) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05284-T 2
EB05284 (0.03µg/ml) staining of Human (A) and Pig (B) Spleen lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05284-T 3
EB05284 (0.05µg/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05284-T 4
EB05284 (1µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05284-T 5
EB05284 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRB2, growth factor receptor-bound protein 2, ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, protein Ash, SH2/SH3 adapter GRB2
Official Symbol: GRB2
Accession Number(s): NP_002077.1; NP_987102.1
Human GeneID(s): 2885
Non-Human GeneID(s): 14784 (mouse), 81504 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Immunogen

Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1; NP_987102.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use at 0.05-0.15µg/ml.
IHC: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2µg/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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