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Goat Anti-GRIK3 / GLUR7 Antibody (EB08683)

Related Products: EBP08683 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08683 Goat Anti-GRIK3 / GLUR7 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08683 1
EB08683(0.3µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: GRIK3, GLUR7, glutamate receptor, ionotropic, kainate 3, EAA5, GLR7, GluR7a, dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)), excitatory amino acid receptor 5, glutamate receptor 7
Official Symbol: GRIK3
Accession Number(s): NP_000822.2
Human GeneID(s): 2899
Non-Human GeneID(s): 14807 (mouse), 298521 (rat)

Immunogen

Peptide with sequence C-KIRQLPIDSDDSRP, from the internal region of the protein sequence according to NP_000822.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Rat, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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