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Goat Anti-GZMA Antibody (EB09312)

Related Products: EBP09312 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09312 Goat Anti-GZMA Antibody Pep-ELISA Yes 100µg specific antibody in 200µl $248.00 Aspiring

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
Click here to download PDF datasheet.

Target Protein

Principal Names: GZMA, granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3), CTLA3, HFSP, CTL tryptase, Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3, Hanukah factor serine protease), granzyme A
Official Symbol: GZMA
Accession Number(s): NP_006135.1
Human GeneID(s): 3001

Immunogen

Peptide with sequence C-QLTEKAKINKY, from the internal region of the protein sequence according to NP_006135.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave bands at approx 37kDa and 20kDa in lysates of cel line Jurkat after 2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 29.0kDa according to NP_006135.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested:
Expected from sequence similarity: Human

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