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Goat Anti-Hap1 (mouse) Antibody (EB07787)

Related Products: EBP07787 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07787 Goat Anti-Hap1 (mouse) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse, Rat $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07787 1
EB07787 (0.1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: Hap1, huntingtin-associated protein 1, RP23-392I3.11, HAP-1, MGC31449
Official Symbol: Hap1
Accession Number(s): NP_034534.1
Non-Human GeneID(s): 15114 (mouse), 29430 (rat)

Immunogen

Peptide with sequence C-QDAHSKRQQKQK, from the C Terminus of the protein sequence according to NP_034534.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100kDa band observed in Mouse and Rat lysates (calculated MW of 66.8kDa according to NP_034534.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Mouse, Rat
Expected from sequence similarity: Mouse, Rat

Specific Reference

This antibody has been successfully used in the following paper:
Md Nabiul Islam, Naoki Maeda, Emi Miyasato, Mir Rubayet Jahan, Abu Md Mamun Tarif, Taiga Ishino, Kanako Nozaki, Koh-Hei Masumoto, Akie Yanai, Koh Shinoda
Expression of huntingtin-associated protein 1 in adult mouse dorsal root ganglia and its neurochemical characterization in reference to sensory neuron subpopulations.
IBRO Rep. 2020 Dec; 9: 258-269.
PMID: 33089002

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