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Goat Anti-HCAP-H Antibody (EB07816)

Related Products: EBP07816 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB07816 Goat Anti-HCAP-H Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB07816 1
EB07816 (0.01µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HCAP-H, NCAPH, non-SMC condensin I complex, subunit H, BRRN1, CAP-H, XCAP-H homolog, barren homolog 1, chromosome-associated protein H, condensin complex subunit 2
Official Symbol: NCAPH
Accession Number(s): NP_056156.2
Human GeneID(s): 23397

Immunogen

Peptide with sequence C-TEDLSDVLVRQGD, from the C Terminus of the protein sequence according to NP_056156.2 .

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110kDa band observed in lysates of cell line K562 (calculated MW of 82.6kDa according to NP_056156.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cabello et al, Mol Biol Cell. 2001 Nov;12(11):3527-37.; PMID: 11694586). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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