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Code Name Applications Availability Size Tested species Price Grade  
EB10176 Goat Anti-HCN3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10176 1
EB10176 (1µg/ml) staining of Rat Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: hyperpolarization activated cyclic nucleotide-gated potassium channel 3, KIAA1535, MGC131493, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3, HCN3
Official Symbol: HCN3
Accession Number(s): NP_065948.1
Human GeneID(s): 57657
Non-Human GeneID(s): 15168 (mouse)

Immunogen

Peptide with sequence C-RGVRGRAPSTGAQ, from the internal region of the protein sequence according to NP_065948.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 85kDa band observed in Rat Kidney lysates (calculated MW of 86.0kDa according to Human NP_065948.1 and 86.8kDa according to Rat NP_446137.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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