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Goat Anti-HEXIM1 Antibody (EB06964)

Related Products: EBP06964 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06964 Goat Anti-HEXIM1 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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EB06964 1
EB06964 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06964 2
EB06964 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB06964 3
EB06964 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1, HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, EDG1, FLJ13562
Official Symbol: HEXIM1
Accession Number(s): NP_006451.1
Human GeneID(s): 10614
Non-Human GeneID(s): 192231 (mouse)

Immunogen

Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 40kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6kDa according to Human NP_006451.1 and 40.2kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse

Specific References

This antibody has been successfully used in the following paper:
Zhijia Wang, Samu V. Himanen, Heidi M. Haikala, Caroline C. Friedel, Anniina Vihervaara, Matjaž Barborič
Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release
(2023) https://doi.org/10.1101/2023.03.21.533608
PMID: 0

This antibody (previous batch) has been successfully used in IP on Human:
Andrii Bugai, Alexandre J.C. Quaresma, Caroline C. Friedel, Tina Lenasi, Christopher R. Sibley, Petra Kukanja, Koh Fujinaga, Melanie Blasius, Thomas Hennig, Jernej Ule, Lars Dölken, Matjaz Barboric
P-TEFb activation by RBM7 shapes a pro-survival transcriptional response to genotoxic stress
Mol Cell. 2019 Apr 18;74(2):254-267.e10.
PMID: 30824372

This antibody (previous batch) has been successfully used in Western blot on Human:
Dalibor Blazek, Fernando Teque, Carl Mackewicz, Matija Peterlin and Jay A. Levy.
The CD8+ cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+ cells.
J Gen Virol. 2016 Jan;97(1):220-4.
PMID: 26499373

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