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Goat Anti-HIP14L / ZDHHC13 Antibody (EB06640)

Related Products: EBP06640 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06640 Goat Anti-HIP14L / ZDHHC13 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06640 1
EB06640 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06640 2
EB06640 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ZDHHC13, HIP14L, HIP3RP, FLJ10852, FLJ10941, MGC64994, zinc finger, DHHC domain containing 13, HIP14-like, HIP14-related protein, huntingtin interacting protein HIP3RP, hypothetical protein similar to ankyrin repeat-containing priotein AKR1, zinc finger, DHHC-type containing 13
Official Symbol: ZDHHC13
Accession Number(s): NP_061901.2; NP_001001483.1
Human GeneID(s): 54503
Non-Human GeneID(s): 243983 (mouse), 365252 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_061901.2 and NP_001001483.1).

Immunogen

Peptide with sequence C-FHPAREKVLRSV, from the C Terminus of the protein sequence according to NP_061901.2; NP_001001483.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 70.9kDa according to NP_061901.2). Recommended concentration: 1-3µg/ml.
IHC: Paraffin embedded Human Lung. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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