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Goat Anti-Histamine receptor H1(internal) Antibody (EB07699)

Code Name Applications Availability Size Tested species Price Grade  
EB07699 Goat Anti-Histamine receptor H1(internal) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping (not applicable to SB-shifix) to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]), fax (888-841-9041), phone (888-320-4628). Please include the following information with your order:

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

Orders are shipped by our US logistics partner Abcore LLC.

 

OR

Order from our local distributor

ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com

EB07699 1
EB07699 (0.5µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB07699 2
EB07699 (4µg/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: histamine receptor H1, H1-R, HRH1, hisH1, histamine receptor, subclass H1
Official Symbol: HRH1
Accession Number(s): NP_000852.1
Human GeneID(s): 3269

Immunogen

Peptide with sequence QQTSVRREDKCETD, from the internal region of the protein sequence according to NP_000852.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 55kDa band observed in Human Heart lysates (calculated MW of 55.8kDa according to NP_000852.1). Recommended concentration: 0.5-1.5µg/ml.
IHC: In paraffin embedded Human Lung shows membranous staining of epitheilial cells in tertiar bronchus and in alveoli. Recommended concentration, 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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