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Goat Anti-HMGB3 / HMG4 Antibody (EB09248)

Related Products: EBP09248 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09248 Goat Anti-HMGB3 / HMG4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09248 1
EB09248 (0.01µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HMGB3, high-mobility group box 3, HMG2A, HMG4, MGC90319, high-mobility group (nonhistone chromosomal) protein 4, non-histone chromosomal protein
Official Symbol: HMGB3
Accession Number(s): NP_005333.2
Human GeneID(s): 3149
Non-Human GeneID(s): 15354 (mouse)

Immunogen

Peptide with sequence C-KFDGAKGPAKVARKK, from the internal region (near C Terminus) of the protein sequence according to NP_005333.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 24kDa band observed in Human Lung lysates (calculated MW of 23.0kDa according to NP_005333.2). Recommended concentration: 0.01-0.03µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Cow

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