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Goat Anti-HMGI-C / HMGA2 Antibody (EB09623)

Related Products: EBP09623 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09623 Goat Anti-HMGI-C / HMGA2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09623 1
EB09623 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: HMGA2, high mobility group AT-hook 2, BABL, HMGI-C, HMGIC, LIPO, STQTL9, High-mobility group protein HMGI-C, high-mobility group (nonhistone chromosomal) protein isoform I-C
Official Symbol: HMGA2
Accession Number(s): NP_003474.1; NP_003475.1
Human GeneID(s): 8091
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003474.1; NP_003475.1) and is not expected to cross-react with HMGA1.

Immunogen

Peptide with sequence C-KAAQKKAEATGEK, from the internal region of the protein sequence according to NP_003474.1; NP_003475.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog

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