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Goat Anti-HMHA1 (aa57-71) Antibody (EB11350)

Related Products: EBP05126 - Immunizing Peptide, EBP11350 - Immunizing Peptide, Goat Anti-HMHA1 / HA-1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB11350 Goat Anti-HMHA1 (aa57-71) Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11350 1
EB11350 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: HA-1, histocompatibility (minor) HA-1, HLA-HA1, KIAA0223, minor histocompatibility antigen HA-1, minor histocompatibility protein HA-1, HMHA1
Official Symbol: ARHGAP45
Accession Number(s): NP_036424.2
Human GeneID(s): 23526
Non-Human GeneID(s): 70719 (mouse), 314618 (rat)
Important Comments: To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)

Immunogen

Peptide with sequence KATGTLKRPTSLSRH-C, from the N Terminus of the protein sequence according to NP_036424.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 200kDa band in lysates of cell lines Jurkat and Molt4 after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kDa according to NP_036424.2. The 200kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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